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DK-MG人胶质母细胞瘤细胞
A07X1788
DK-MG
货号 纯度 规格 目录价 会员价 库存 数量 购买
A07X1788 1×106cells/T25 1×106cells/T25 ¥30000.00 登录查看
产品详情

Cell line:    DK-MG
DSMZ no.:A07X1788
Species:    human (Homo sapiens)
Cell type:    glioblastoma
Origin:    established from the glioblastoma multiforme (right parietal, at diagnosis) of a 67-year-old woman in 1991
Reference(s):    14689
Biosafety level:    1
Permissions and restrictions:    A
     DSMZ Cell Culture Data:
Morphology:    spindle, fibroblast-like cells growing mostly adherent as monolayer; image; image
Medium:    80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine
Subculture:    seed out at ca. 1.0 x 106 cells/80 cm2; split nearly confluent culture ca. 1:5 once a week days using trypsin/EDTA
Incubation:    at 37 °C with 5% CO2
Doubling time:    ca. 70-80 hours
Harvest:    cell harvest of ca. 4-5 x 106 cells/80 cm2
Storage:    frozen with 70% medium, 20% FBS, 10% DMSO
     DSMZ Scientific Data:
Mycoplasma:    negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:    To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

Authenticity: STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics: human hypodiploid karyotype with 12% polyploidy - 44(42-44)<2n>XX, +7, -9, -10, -12, -14, +mar, del(6)(q13q25), add(9)(p2?2), der(15)t(14;15)(q12;p11), del(16)(p13), del(17)(q25), add(19)(q1?3), variable numbers of dmin detectible in most cells - resembles published karyotype
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