p70 S6 Kinase (12D4) Rabbit Monoclonal Antibody - 上海抚生实业有限公司

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p70 S6 Kinase (12D4) Rabbit Monoclonal Antibody

产品编号： A-DK11560

英文名称： p70 S6 Kinase (12D4) Rabbit Monoclonal Antibody

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货号	纯度	规格	目录价	会员价	库存	数量	购买
A-DK11560-50µl	50µl	50µl	¥1200.00	登录查看
A-DK11560-100µl	100µl	100µl	¥2200.00	登录查看

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资料/datasheet

产品名称	p70 S6 Kinase (12D4) Rabbit Monoclonal Antibody
英文名称	p70 S6 Kinase (12D4) Rabbit Monoclonal Antibody
类别	抗原抗体
反应种属	Human,Mouse,Rat
应用	WB,IHC-P,IHC-F,IF-ICC,IF-P,IF-F,IP,FCM

别名	EC 2.7.11.1; KS6B1; P70-S6K; RPS6KB1; Ribosomal protein S6 kinase, Ribosomal protein S6 kinase 70kDa polypeptide 1; S6K1; kinase p70S6K; p70-S6K;
Clonality	Monoclonal
存储缓冲液	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
稀释度	WB 1:1000-1:2000,IHC-P/IHC-F 1:100-1:200,IF-ICC 1:50-1:200,IF-P/IF-F 1:50-1:200,IP 1:20-1:50,FCM 1:20-1:100
运输及保存条件	20°C/1 year
Human Gene ID	6198
预测分子量	59kDa
参考分子量	70kDa
蛋白名称	STK14A
基因名称	RPS6KB1
宿主	Rabbit
同种型	LgG
组织表达	Widely expressed..
细胞定位	Cell junction, synapse, synaptosome. Mitochondrion outer membrane. Mitochondrion Note=Colocalizes with URI1 at mitochond rion [Isoform Alpha II]: Cytoplasm.
注意事项	p70 S6 Kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
功能	Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promo te cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B , RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nut rient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, pho sphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activa tion. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF 2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer r ound of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phospho rylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regu lation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates ce ll survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mit ochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RP S6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates T NF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradati on of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved i n cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme C AD, downstream of MTOR (PubMed:11500364, PubMed:12801526, PubMed:14673156, PubMed:15071500, PubMed::1628600 6, PubMed:17052453, PubMed:17053147, PubMed:17936702, PubMed:18952604, PubMed:19085255, , PubMed:19935711, P ubMed:19995915, PubMed:23429703). Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition (PubMed:28178239).

Western blot analysis of p70 S6 Kinase expression in 293T cell lysate.

p70 S6 kinase is a mitogen activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression.Phosphorylation of Thr229 in the catalytic domain and Thr389 in the linker domain are most critical for kinase function. Phosphorylation of Thr389, however, most closely correlates with p70 kinase activity in vivo. Prior phosphorylation of Thr389 is required for the action of phosphoinositide 3-dependent protein kinase 1 (PDK1) on Thr229.