存储缓冲液
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Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C
short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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细胞定位
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Cytoplasm. Secreted. Nucleus Note=Secretion occurs in response to oxidative stress in vascular smooth muscle through a v
esicular secretory pathway that includes Rho GTPase signaling, actin remodeling, and myosin II activation
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功能
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Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:, PubMed:20676357, Pu
bMed:21245143, PubMed:25678563, PubMed:21593166). Exerts a strong chemotactic effect on leukocytes partly through activatio
n of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation (PubMed
:11943775, PubMed:21245143). Activates endothelial cells (ECs) in a proinflammatory manner by stimulating activation of NF-
kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1 (PubMed:
15130913). Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in E
C chemotaxis and apoptosis (PubMed:31063815). In response to oxidative stress, initiates proapoptotic and antiapoptotic sig
naling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2 (PubMed:23180369). Nega
tively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5
/ASK1 (PubMed:26095851). Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes
and regulates binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in
clearance of protein aggregates (PubMed:25678563). Plays an important role in platelet activation and aggregation (By simi
larity). Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as w
ell as by facilitating the interaction between integrin and the cell cytoskeleton (By similarity). Binds heparan sulfate gl
ycosaminoglycans (PubMed:11943775). Inhibits replication of influenza A virus (IAV) (PubMed:19207730). Inhibits ITCH/AIP4-m
ediated ubiquitination of matrix protein 1 (M1) of IAV by impairing the interaction of ITCH/AIP4 with M1, followed by the s
uppression of the nuclear export of M1, and finally reduction of the replication of IAV (PubMed:30328013, PubMed:22347431).
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