存储缓冲液
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Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C
short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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稀释度
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WB 1:1000-1:2000,IHC-P/IHC-F 1:100-1:200,IF-ICC 1:50-1:200,IF-P/IF-F 1:50-1:200,IP 1:20-1:50
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组织表达
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Expressed in adult spleen, liver, and kidney (PubMed:9781816). Expressed in fetal thymus, liver, placenta, spleen, lung,
and kidney but not brain (PubMed:9781816). Expressed in macrophages (PubMed:29038465).
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细胞定位
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Cytoplasm. Nucleus. Secreted. Note=Cytoplasmic NMI localizes in punctate granular structures (PubMed:9781816, PubMed:109
50963). Nuclear localization increased following IFN-alpha treatment (PubMed:9781816, PubMed:10950963). Extracelullar fo
llowing secretion by macrophage (PubMed:29038465).
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功能
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Acts as a signaling pathway regulator involved in innate immune system response (PubMed:, PubMed:26342464
, PubMed:29038465, PubMed:29350881). In response to interleukin 2/IL2 and interferon IFN-gamma/IFNG, interacts with signal
transducer and activator of transcription/STAT which activate the transcription of downstream genes involved in a multitude
of signals for development and homeostasis (PubMed:9989503). Enhances the recruitment of CBP/p300 coactivators to STAT1 an
d STAT5, resulting in increased STAT1- and STAT5-dependent transcription (PubMed:9989503). In response to interferon IFN-al
pha, associates in a complex with signaling pathway regulator IFI35 to regulate immune response; the complex formation prev
ents proteasome-mediated degradation of IFI35 (PubMed:10779520, PubMed:10950963). In complex with IFI35, inhibits virus-tri
ggered type I IFN-beta production when ubiquitinated by ubiquitin-protein ligase TRIM21 (PubMed:26342464). In complex with
IFI35, negatively regulates nuclear factor NF-kappa-B signaling by inhibiting the nuclear translocation, activation and tra
nscription of NF-kappa-B subunit p65/RELA, resulting in the inhibition of endothelial cell proliferation, migration and re-
endothelialization of injured arteries (PubMed:29350881). Negatively regulates virus-triggered type I interferon/IFN produc
tion by inducing proteosome-dependent degradation of IRF7, a transcriptional regulator of type I IFN, thereby interfering w
ith cellular antiviral responses (By similarity). Beside its role as an intracellular signaling pathway regulator, also fun
ctions extracellularly as damage-associated molecular patterns (DAMPs) to promote inflammation, when actively released by m
acrophage to the extracellular space during cell injury or pathogen invasion (PubMed:29038465). Macrophage-secreted NMI act
ivates NF-kappa-B signaling in adjacent macrophages through Toll-like receptor 4/TLR4 binding and activation, thereby induc
ing NF-kappa-B translocation from the cytoplasm into the nucleus which promotes the release of proinflammatory cytokines (P
ubMed:29038465).
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