
产品名称
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ANAPC2 Rabbit Polyclonal Antibody (C-term)
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英文名称
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ANAPC2 Rabbit Polyclonal Antibody (C-term)
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类别
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抗体产品
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反应种属
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Human, Mouse
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应用
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WB, IHC-P, IF-ICC
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别名
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Anaphase-promoting complex subunit 2, APC2, Cyclosome subunit 2, ANAPC2, APC2, KIAA1406
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Clonality
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Polyclonal
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稀释度
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IF-ICC 1:25, IHC-P 1:100-500, WB 1:1000
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预测分子量
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94kDa
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宿主
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Rabbit
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同种型
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IgG
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特异性
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This ANAPC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide be
tween 712-746 amino acids from the C-terminal region of human ANAPC2.
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背景介绍
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Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls pro
gression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequ
ent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lowe
r extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. The CDC20-APC/C complex positively regulates
the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/
C-induced degradation of NEUROD2 drives presynaptic differentiation.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ANAPC2 with staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor® 555
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Immunohistochemical analysis of paraffin-embedded H. thymus section using ANAPC2 Antibody (C-term). staining.
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Western blot analysis of lysate from human testis tissue lysate, using ANAPC2 Antibody (C-term). 149 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.