Cell line: DK-MG
DSMZ no.:A07X1788
Species: human (Homo sapiens)
Cell type: glioblastoma
Origin: established from the glioblastoma multiforme (right parietal, at diagnosis) of a 67-year-old woman in 1991
Reference(s): 14689
Biosafety level: 1
Permissions and restrictions: A
DSMZ Cell Culture Data:
Morphology: spindle, fibroblast-like cells growing mostly adherent as monolayer; image; image
Medium: 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine
Subculture: seed out at ca. 1.0 x 106 cells/80 cm2; split nearly confluent culture ca. 1:5 once a week days using trypsin/EDTA
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 70-80 hours
Harvest: cell harvest of ca. 4-5 x 106 cells/80 cm2
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
| Authenticity: |
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: |
human hypodiploid karyotype with 12% polyploidy - 44(42-44)<2n>XX, +7, -9, -10, -12, -14, +mar, del(6)(q13q25), add(9)(p2?2), der(15)t(14;15)(q12;p11), del(16)(p13), del(17)(q25), add(19)(q1?3), variable numbers of dmin detectible in most cells - resembles published karyotype |
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