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K63-linkage Specific Ubiquitin (4W15) Rabbit Monoclonal Antibody
A-DK9737
K63-linkage Specific Ubiquitin (4W15) Rabbit Monoclonal Antibody
货号 纯度 规格 目录价 会员价 库存 数量 购买
A-DK9737-50µl 50µl 50µl ¥1200.00 登录查看
A-DK9737-100µl 100µl 100µl ¥2200.00 登录查看
产品详情

产品名称

K63-linkage Specific Ubiquitin (4W15) Rabbit Monoclonal Antibody

英文名称

K63-linkage Specific Ubiquitin (4W15) Rabbit Monoclonal Antibody

类别

抗原抗体

反应种属

Human,Mouse,Rat

应用

WB,IHC-P,FCM,IF-P

别名

FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A

Clonality

Monoclonal

存储缓冲液

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% New type preservative N and 0.05% BSA.

免疫原

A synthetic peptide of human Ubiquitin (linkage-specific K63)

稀释度

WB 1:1000, IHC-P/IF-P 1:200-1:500, FCM 1:200

预测分子量

26kDa

运输及保存条件

20°C/1 year

Human Gene ID

7314

基因名称

UBB

蛋白名称

UBCEP1

宿主

Rabbit

同种型

LgG

细胞定位

[Ubiquitin]: Cytoplasm. Nucleus

注意事项

K63-linkage Specific Ubiquitin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

功能

[Ubiquitin]: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjuga
ted to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residue
s of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiqu
itin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue
of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic r
eticulum-associated degradation) and in cell- cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-li
nked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked i
s involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription
factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usua
lly conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observ
ed. When polyubiquitin is free (unanchored- polyubiquitin), it also has distinct roles, such as in activation of protein kina
ses, and in signaling.

                           

Western blot detection of Ubiquitin (linkage-specific K63) in Hela,A549 cell lysates using Ubiquitin (linkage-specific K63) antibody(1:1000 diluted).

Plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue.

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