
产品名称
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GSTO1 Rabbit Polyclonal Antibody (Center)
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英文名称
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GSTO1 Rabbit Polyclonal Antibody (Center)
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类别
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抗原抗体
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反应种属
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Human
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应用
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WB, IHC-P, FCM
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别名
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Glutathione S-transferase omega-1, GSTO-1, Glutathione S-transferase omega 1-1, GSTO 1-1, Glutathione-dependent dehydroa
scorbate reductase, Monomethylarsonic acid reductase, MMA(V) reductase, S-(Phenacyl)glutathione reductase, ,
SPG-R, GSTO1GSTTLP28
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Clonality
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Polyclonal
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稀释度
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WB 1:1000, IHC-P 1:100-500, FCM 1:10-50
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预测分子量
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28kDa
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宿主
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Rabbit
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Human Gene ID
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NP_001177931.1
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同种型
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IgG
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组织表达
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Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart Low
est expression in brain, placenta and lung
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细胞定位
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Cytoplasm, cytosol.
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背景介绍
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GSTO1 is a member of the theta class glutathione S-transferase-like (GSTTL) protein family. In mouse, this protein acts
as a small stress response protein, likely involved in cellular redox homeostasis.
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纯化
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Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammon
ium Sulfate (SAS) precipitation followed by dialysis against PBS.
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特异性
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This GSTO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-155 amino acid
s from the Central region of human GSTO1.
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Anti-GSTO1 Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/
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Western blot analysis of GSTO1 Antibody (Center) in NCI-H460 cell line lysates (35ug/lane). GSTO1 (arrow) was detected using the purified Pab.
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Foalin-fixed and paraffin-embedded human skeletal muscle reacted with GSTO1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; c
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GSTO1 Antibody (Center) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of GSTO1 (arrow) using rabbit polyclonal GSTO1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GSTO1 gene.