别名
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Tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, Kinase EMT, Kinase TLK, T-cell-specific kinase, Itk, Emt,
Tlk, Tsk
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Clonality
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Polyclonal
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稀释度
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WB 1:1000
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预测分子量
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72kDa
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宿主
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Rabbit
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同种型
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IgG
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组织表达
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Is detected in the thymus, lymph node and very faintly in the spleen, but is not detected in the liver, lung, kidney, he
art, brain, intestine or testis. Expressed in T-lymphocytes and mast cells. It may also be expressed in natural killer c
ells
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细胞定位
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Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimu
lation.
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特异性
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This Mouse Itk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide
between 156-190 amino acids from the N-terminal region of human Mouse Itk.
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背景介绍
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Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, func
tion and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activat
e T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity o
f the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full
activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its
substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-c
ells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins:
the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are re
cruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.
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