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Mouse Itk Rabbit Polyclonal Antibody (N-term)
A-DK14598
Mouse Itk Rabbit Polyclonal Antibody (N-term)
货号 纯度 规格 目录价 会员价 库存 数量 购买
A-DK14598-50μl 50μl 50μl ¥1100.00 登录查看
A-DK14598-100μl 100μl 100μl ¥2000.00 登录查看
产品详情

产品名称

Mouse Itk Rabbit Polyclonal Antibody (N-term)

英文名称

Mouse Itk Rabbit Polyclonal Antibody (N-term)

类别

抗体产品

反应种属

Human, Mouse

应用

WB

别名

Tyrosine-protein kinase ITK/TSK, IL-2-inducible T-cell kinase, Kinase EMT, Kinase TLK, T-cell-specific kinase, Itk, Emt,

Tlk, Tsk

Clonality

Polyclonal

稀释度

WB 1:1000

预测分子量

72kDa

宿主

Rabbit

同种型

IgG

组织表达

Is detected in the thymus, lymph node and very faintly in the spleen, but is not detected in the liver, lung, kidney, he

art, brain, intestine or testis. Expressed in T-lymphocytes and mast cells. It may also be expressed in natural killer c

ells

细胞定位

Cytoplasm. Nucleus. Note=Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimu

lation.

特异性

This Mouse Itk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide

between 156-190 amino acids from the N-terminal region of human Mouse Itk.

背景介绍

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, func
tion and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activat
e T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity o
f the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full
activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its
substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-c
ells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins:
the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are re
cruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.

                           

Western blot analysis of lysates from mouse thymus and mouse spleen tissue lysate (from left to right), using Mouse Itk Antibody (N-term). 982 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary

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