
产品名称
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GSTM1 Rabbit Polyclonal Antibody (C-term)
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英文名称
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GSTM1 Rabbit Polyclonal Antibody (C-term)
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类别
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抗体产品
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反应种属
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Human, Mouse
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应用
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IHC-P, FCM, WB
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别名
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Glutathione S-transferase Mu 1, GST HB subunit 4, GST class-mu 1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GSTM1, GST1
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Clonality
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Polyclonal
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稀释度
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WB 1:1000, IHC-P 1:100-500, FCM 1:10-50
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预测分子量
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26kDa
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宿主
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Rabbit
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Human Gene ID
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NP_000169.1
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运输及保存条件
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Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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同种型
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IgG
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功能
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Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the
formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2) (PubMed:9084911). Participa
tes in the formation of novel hepoxilin regioisomers (PubMed:21046276).
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组织表达
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Liver (at protein level).
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细胞定位
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Cytoplasm.
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特异性
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This GSTM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-211 amino ac
ids from the C-terminal region of human GSTM1.
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背景介绍
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GSTM1 is a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification
of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress
, by conjugation with glutathione. The mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are kno
wn to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins a
s well as affect the toxicity and efficacy of certain drugs.
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Western blot analysis of lysates from Jurkat, U251 cell line and human liver tissue (from left to right), using GSTM1 Antibody (C-term) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GSTM1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; N
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GSTM1 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.