MEK1 Rabbit Polyclonal Antibody (S218/222) - 上海抚生实业有限公司

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MEK1 Rabbit Polyclonal Antibody (S218/222)

产品编号： A-DK16133

英文名称： MEK1 Rabbit Polyclonal Antibody (S218/222)

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货号	纯度	规格	目录价	会员价	库存	数量	购买
A-DK16133-50μl	50μl	50μl	¥1100.00	登录查看
A-DK16133-100μl	100μl	100μl	¥2000.00	登录查看

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资料/datasheet

产品名称	MEK1 Rabbit Polyclonal Antibody (S218/222)
英文名称	MEK1 Rabbit Polyclonal Antibody (S218/222)
类别	抗体产品
反应种属	Human, Rat, Mouse
应用	WB

别名	Dual specificity mitogen-activated protein kinase kinase 1, MAP kinase kinase 1, MAPKK 1, MKK1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK 1, MAP2K1, MEK1, PRKMK1
Clonality	Polyclonal
稀释度	WB 1:1000
预测分子量	43kDa
宿主	Rabbit
Human Gene ID	NP_002746.1
运输及保存条件	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
同种型	IgG
功能	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK 2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphoryla tion of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular intera ction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF act ivation (PubMed:29433126). Depending on the cellular context, this pathway mediates diverse biological functions such as ce ll growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cy toskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus . The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus durin g mitosis.
组织表达	Widely expressed, with extremely low levels in brain.
细胞定位	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing cent er, spindle pole body. Cytoplasm. Nucleus Membrane; Peripheral membrane protein. Note=Localizes at centrosomes during pr ometaphase, midzone during anaphase and midbody during telophase/cytokinesis (PubMed:14737111). Membrane localization is probably regulated by its interaction with KSR1 (PubMed:10409742)
特异性	This MEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-234 amino aci ds from human MEK1.
背景介绍	MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase ki nase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple bi ochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases up on wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, t his kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and deve lopment.

Western blot analysis of lysate from rat C6 cell line, using MEK1 Antibody (S218/222)at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Western blot analysis of anti-MEK1 Antibody (S218/222) in A2058, A375 cell line lysates (35ug/lane). MEK1 (arrow) was detected using the purified Pab.