MDM2 (1H9) Mouse Monoclonal Antibody - 上海抚生实业有限公司

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MDM2 (1H9) Mouse Monoclonal Antibody

产品编号： A-DK16612

英文名称： MDM2 (1H9) Mouse Monoclonal Antibody

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货号	纯度	规格	目录价	会员价	库存	数量	购买
A-DK16612-50μl	50μl	50μl	¥1100.00	登录查看
A-DK16612-100μl	100μl	100μl	¥2000.00	登录查看

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产品名称	MDM2 (1H9) Mouse Monoclonal Antibody
英文名称	MDM2 (1H9) Mouse Monoclonal Antibody
类别	抗体产品
反应种属	Human
应用	WB

别名	E3 ubiquitin-protein ligase Mdm2, 6.3.2.-, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2,
Clonality	Monoclonal
稀释度	WB 1:4000
预测分子量	55kDa
宿主	Mouse
运输及保存条件	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
同种型	IgG1,κ
功能	E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibit s p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also ac ts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitin ation and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination a nd subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal de gradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, , PubMed:190 98711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, , PubMed:2212891 1). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule ce lls (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor end ocytosis (By similarity). Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative str ess, and commitment to the mitochondrial pathway of apoptosis (PubMed:30879903). Binds NDUFS1 leading to its cytosolic rete ntion rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I a nd complex III), decreased complex I activity, ROS production, and apoptosis (PubMed:30879903).
组织表达	Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform M dm2-G are observed in a range of cancers but absent in normal tissues
细胞定位	Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Nucleus. Note=Expressed predominantly in the nucleoplasm. Interacti on with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in bo th ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF 1 isoform A in the nucleus
特异性	This MDM2 antibody is generated from a mouse immunized with a recombinant protein between 196-435 amino acids from the h uman MDM2.
背景介绍	"E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibit s p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also ac ts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitin ation and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 which links growth factor and DNA damage response pathways. Mediates ubiquitination a nd subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal de gradation.

Anti-MDM2 Antibody at 1:4000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.