
产品名称
|
ATP6V1B2 (2E13) Mouse Monoclonal antibody
|
英文名称
|
ATP6V1B2 (2E13) Mouse Monoclonal antibody
|
类别
|
抗体产品
|
反应种属
|
Human,Mouse,Rat
|
应用
|
IHC-P,IHC-F,WB
|

基因名称
|
ATP6V1B2
|
宿主
|
Mouse
|
存储缓冲液
|
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
|
免疫原
|
Full length human recombinant protein of human ATP6V1B2 (NP_001684) produced in HEK293T cell.
|
稀释度
|
WB 1:2000, IHC-P/IHC-F 1:150
|
预测分子量
|
56.3kDa
|
运输及保存条件
|
-20°C/1 year
|
同种型
|
IgG2a
|
背景介绍
|
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic
intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein
sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is com
posed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits,
as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is o
ne of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 20
08]
|
纯化
|
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
|

 Mouse Monoclonal antibody.jpg)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ATP6V1B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C
 Mouse Monoclonal antibody.jpg)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1B2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
 Mouse Monoclonal antibody.jpg)
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATP6V1B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C
 Mouse Monoclonal antibody.jpg)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ATP6V1B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C
 Mouse Monoclonal antibody.jpg)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-ATP6V1B2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C