
产品名称
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KLF5 (7S4) Mouse Monoclonal antibody
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英文名称
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KLF5 (7S4) Mouse Monoclonal antibody
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类别
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抗体产品
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反应种属
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Human,Mouse,Rat
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应用
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WB
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基因名称
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KLF5
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宿主
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Mouse
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存储缓冲液
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PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
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免疫原
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Human recombinant protein fragment corresponding to amino acids 1 - 457 of human KLF5 (NP_001721) produced in E.coli.
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稀释度
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WB 1:500-2000
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预测分子量
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50.6kDa
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运输及保存条件
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-20°C/1 year
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同种型
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IgG1
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背景介绍
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This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcripti
onal activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downst
ream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both
promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and i
n cardiovascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
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纯化
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Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
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 Mouse Monoclonal antibody.jpg)
Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-KLF5 monoclonal antibody. (1:2000) (1:500)
 Mouse Monoclonal antibody.jpg)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLF5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLF5. Positive