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MDM2 (9Z19) Mouse Monoclonal antibody
A-DK18317
MDM2 (9Z19) Mouse Monoclonal antibody
货号 纯度 规格 目录价 会员价 库存 数量 购买
A-DK18317-50μl 50μl 50μl ¥1200.00 登录查看
A-DK18317-100μl 100μl 100μl ¥2200.00 登录查看
产品详情

产品名称

MDM2 (9Z19) Mouse Monoclonal antibody

英文名称

MDM2 (9Z19) Mouse Monoclonal antibody

类别

抗体产品

反应种属

Human

应用

IHC-P,IHC-F,WB

基因名称

MDM2

宿主

Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 119-438 of human MDM2 (NP_002383) produced in E.coli.

稀释度

WB 1:2000, IHC-P/IHC-F 1:150

预测分子量

55.8kDa

运输及保存条件

-20°C/1 year

同种型

IgG1

背景介绍

This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein tha
t binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpressio
n of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This pro
tein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects
the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and riboso
mal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and norma
l tissues. [provided by RefSeq, Jul 2008]

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

                           

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MDM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min
                           
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM2. Positive
                             

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