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PSMA6 (Proteasome 20S alpha 6) (9G17) Mouse Monoclonal antibody
A-DK18523
PSMA6 (Proteasome 20S alpha 6) (9G17) Mouse Monoclonal antibody
货号 纯度 规格 目录价 会员价 库存 数量 购买
A-DK18523-50μl 50μl 50μl ¥1200.00 登录查看
A-DK18523-100μl 100μl 100μl ¥2200.00 登录查看
产品详情

产品名称

PSMA6 (Proteasome 20S alpha 6) (9G17) Mouse Monoclonal antibody

英文名称

PSMA6 (Proteasome 20S alpha 6) (9G17) Mouse Monoclonal antibody

类别

抗体产品

反应种属

Human,Dog,Rat,Monkey,Mouse

应用

FCM,WB

基因名称

PSMA6

宿主

Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PSMA6 (NP_002782) produced in E.coli.

稀释度

WB 1:500-1000, FCM 1:100

预测分子量

27.2kDa

运输及保存条件

-20°C/1 year

同种型

IgG1

背景介绍

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structur
e is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7
beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP
/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome
, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha
subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

                           

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

                           

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMA6 antibody, and then analyzed by flow cytometry.

                             

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6. Positive                             

 

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