
产品名称
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PSMA6 (Proteasome 20S alpha 6) (9G17) Mouse Monoclonal antibody
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英文名称
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PSMA6 (Proteasome 20S alpha 6) (9G17) Mouse Monoclonal antibody
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类别
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抗体产品
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反应种属
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Human,Dog,Rat,Monkey,Mouse
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应用
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FCM,WB
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基因名称
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PSMA6
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宿主
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Mouse
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存储缓冲液
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PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
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免疫原
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Full length human recombinant protein of human PSMA6 (NP_002782) produced in E.coli.
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稀释度
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WB 1:500-1000, FCM 1:100
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预测分子量
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27.2kDa
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运输及保存条件
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-20°C/1 year
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同种型
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IgG1
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背景介绍
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The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structur
e is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7
beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP
/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome
, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha
subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq]
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纯化
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Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
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 (9G17) Mouse Monoclonal antibody.jpg)
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
 (9G17) Mouse Monoclonal antibody.jpg)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMA6 antibody, and then analyzed by flow cytometry.
 (9G17) Mouse Monoclonal antibody.jpg)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA6. Positive